Quote:
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Originally Posted by dan_rose
Anyone got access? I'd like to know what EODF actually means in terms of hours/calories in this study.
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"Mice were randomly grouped to ad libitum (AL) group and every other day fasting (EODF) group. All mice were co-housed (2 mice per
cage) on chow diet or single housed on HFD for 2 weeks prior to study initiation to allow for acclimation to the animal facility and were
then placed on chow diet or HFD. Under the chow diet, the AL group mice were allowed unrestricted access to food, while EODF
groups were fed with alternating 24 hr periods (15 cycles for long-term study and 3 cycles for short-term study) of free access to
food followed by 24 hr fasting. Under the HFD, mice were allowed unrestricted access to HFD for 3 months to induce an obese
phenotype then randomly separated into AL and EODF groups and subjected to the AL or EODF feeding regimen (15 cycles). For
EODF at thermoneutrality, mice were acclimatized to thermoneutrality (30C) for 1 day and then treated either AL or EODF over
the entire period at thermoneutrality (30C) condition. Body weight was measured daily. Food intake was calculated daily quantifying
the weight of the remaining food. Data were normalized to the body weight of the mice."