Ironjustic
Sun, May-25-08, 17:15
ORIGINAL RESEARCH ARTICLE Daily Supplementation with Iron
Increases Lipid Peroxidation in Young Women with Low Iron
Stores Sarah M. King*,1, Carmen M. Donangelo, Mitchell D.
Knutson*,2, Patrick
B. Walter,3, Bruce N. Ames,3, Fernando E. Viteri*,4,3 and
Janet C. King,3
* Department of Nutritional Sciences and Toxicology,
University of California, Berkeley, California 94720;
Laborat=F3rio de Bioqu=EDmica Nutricional e de Alimentos,
Departamento de Bioquimica, Instituto de Quimica,
Universidade Federal do Rio de Janeiro, 21949=96900 Rio de
Janeiro, Brazil; Department of Molecular and Cell Biology,
University of California, Berkeley, California 94720; and
USDA Western Human Nutrition Research Center, Davis,
California 95616
To whom requests for reprints should be addressed at 4
Children=92s Hospital Oakland Research Institute, 5700 Martin
Luther King Jr. Way, Oakland, CA 94609. E-mail
fviteri@chori.org
The aim of this study was to determine whether women with low
iron stores (plasma ferritin 20 =B5g/L) receiving a daily iron
supplement for 8 wks at a level commonly used to treat poor
iron status develop increased lipid peroxidation as measured
by ethane exhalation rates and plasma malondialdehyde. The
women served as their own control as pre- and
post-supplementation periods were compared. Twelve women
participated in the study for a 70-day period and consumed
daily iron supplements (98 mg of iron as ferrous sulfate) from
day 14 to day 70. Baseline blood and expired air samples were
obtained on days 1 and 14; measurements during supplementation
were performed on days 56 and 70, that is at 6 and 8 weeks of
supplementation. Iron status improved during the iron
supplementation period; biochemical indicators of lipid
peroxidation also increased. After 6 wks of iron
supplementation, serum ferritin almost doubled and body iron
more than doubled. Hemoglobin levels increased slightly and
other indicators of iron status became normal. However, plasma
malondialdehyde (MDA) and breath ethane exhalation rates
(BEER) increased by more than 40% between baseline and 6 wks
of supplementation; these increases correlated significantly
with plasma iron and ferritin levels. MDA was positively
correlated with BEER. BEER increased further after 8 wks of
iron supplementation. The increased indicators of lipid
peroxidation with duration of supplementation and as iron
status improved suggest that providing daily nearly 100 mg
iron may not be a totally innocuous regimen for correcting
iron depletion in women.
Key Words: iron =95 supplements =95 malondialdehyde =95 breath
ethane =95 lipid peroxidation =95 oxidative stress =95 iron
status =95 women First published online April 11, 2008
Experimental Biology and Medicine 233:701-707 (2008) doi:
10.3181/0708-RM-233 =A9 2008 by the Society for Experimental
Biology and Medicine
Who loves ya. Tom
Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
Increases Lipid Peroxidation in Young Women with Low Iron
Stores Sarah M. King*,1, Carmen M. Donangelo, Mitchell D.
Knutson*,2, Patrick
B. Walter,3, Bruce N. Ames,3, Fernando E. Viteri*,4,3 and
Janet C. King,3
* Department of Nutritional Sciences and Toxicology,
University of California, Berkeley, California 94720;
Laborat=F3rio de Bioqu=EDmica Nutricional e de Alimentos,
Departamento de Bioquimica, Instituto de Quimica,
Universidade Federal do Rio de Janeiro, 21949=96900 Rio de
Janeiro, Brazil; Department of Molecular and Cell Biology,
University of California, Berkeley, California 94720; and
USDA Western Human Nutrition Research Center, Davis,
California 95616
To whom requests for reprints should be addressed at 4
Children=92s Hospital Oakland Research Institute, 5700 Martin
Luther King Jr. Way, Oakland, CA 94609. E-mail
fviteri@chori.org
The aim of this study was to determine whether women with low
iron stores (plasma ferritin 20 =B5g/L) receiving a daily iron
supplement for 8 wks at a level commonly used to treat poor
iron status develop increased lipid peroxidation as measured
by ethane exhalation rates and plasma malondialdehyde. The
women served as their own control as pre- and
post-supplementation periods were compared. Twelve women
participated in the study for a 70-day period and consumed
daily iron supplements (98 mg of iron as ferrous sulfate) from
day 14 to day 70. Baseline blood and expired air samples were
obtained on days 1 and 14; measurements during supplementation
were performed on days 56 and 70, that is at 6 and 8 weeks of
supplementation. Iron status improved during the iron
supplementation period; biochemical indicators of lipid
peroxidation also increased. After 6 wks of iron
supplementation, serum ferritin almost doubled and body iron
more than doubled. Hemoglobin levels increased slightly and
other indicators of iron status became normal. However, plasma
malondialdehyde (MDA) and breath ethane exhalation rates
(BEER) increased by more than 40% between baseline and 6 wks
of supplementation; these increases correlated significantly
with plasma iron and ferritin levels. MDA was positively
correlated with BEER. BEER increased further after 8 wks of
iron supplementation. The increased indicators of lipid
peroxidation with duration of supplementation and as iron
status improved suggest that providing daily nearly 100 mg
iron may not be a totally innocuous regimen for correcting
iron depletion in women.
Key Words: iron =95 supplements =95 malondialdehyde =95 breath
ethane =95 lipid peroxidation =95 oxidative stress =95 iron
status =95 women First published online April 11, 2008
Experimental Biology and Medicine 233:701-707 (2008) doi:
10.3181/0708-RM-233 =A9 2008 by the Society for Experimental
Biology and Medicine
Who loves ya. Tom
Jesus Was A Vegetarian! http://tinyurl.com/2r2nkh
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk