Ironjustic
Wed, Mar-12-08, 17:15
Comparison of hepatic oxidative DNA damage in patients with
chronic hepatitis B and C. [JOURNAL ARTICLE] J Viral Hepat
2008 Mar 6. Fujita N, Sugimoto R, Ma N, Tanaka H, Iwasa M,
Kobayashi Y, Kawanishi S, Watanabe S, Kaito M, Takei Y
8-Hydroxydeoxyguanosine (8-OHdG) is a promutagenic DNA lesion
produced by hydroxyl radicals and is recognized as a useful
marker in estimating DNA damage induced by oxidative stress.
The aim of this study was to clarify the clinical significance
of hepatic 8-OHdG levels in patients with chronic viral
hepatitis. Hepatic 8-OHdG accumulation was investigated in
patients with chronic hepatitis C (CH-
C) (n= 77) and chronic hepatitis B (CH-B) (n = 34) by
immunohistochemical staining of liver biopsy samples.
8-OHdG positive hepatocytes were significantly higher in
patients with CH-C compared to CH-B (median 55.0 vs 18.8
cells/10(5) mum(2), P < 0.0001). The number of positive
hepatocytes significantly increased with the elevation
of serum aminotransferase levels, especially in CH-C
patients (8-OHdG vs alanine aminotransferase
(ALT)/aspartate aminotrasferase (AST) were r =
0.738/0.720 in CH-C and 0.506/0.515 in CH-B). 8-OHdG
reactivity was strongly correlated with body and hepatic
iron storage markers in CH-C (vs serum ferritin, r =
0.615; vs hepatic total iron score, r = 0.520; vs
hepatic hepcidin mRNA levels, r = .571), although it was
related to serum HBV-DNA titers (r = 0.540) and age of
patients (r = -0.559) in CH-B. These results indicate
that hepatic oxidative DNA damage is common in chronic
viral hepatitis, in particular chronic HCV-infected
patients, suggesting a possible link between chronic
hepatic inflammation and hepatocarcinogenesis. The
strong positive correlation between hepatic DNA damage
and iron overload suggests that iron content is one of
the most likely mediators of hepatic oxidative stress
and iron reduction may be beneficial to reduce the
incidence of hepatic cancer in CH-C patients. Journal of
viral hepatitis [J Viral Hepat]
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Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
chronic hepatitis B and C. [JOURNAL ARTICLE] J Viral Hepat
2008 Mar 6. Fujita N, Sugimoto R, Ma N, Tanaka H, Iwasa M,
Kobayashi Y, Kawanishi S, Watanabe S, Kaito M, Takei Y
8-Hydroxydeoxyguanosine (8-OHdG) is a promutagenic DNA lesion
produced by hydroxyl radicals and is recognized as a useful
marker in estimating DNA damage induced by oxidative stress.
The aim of this study was to clarify the clinical significance
of hepatic 8-OHdG levels in patients with chronic viral
hepatitis. Hepatic 8-OHdG accumulation was investigated in
patients with chronic hepatitis C (CH-
C) (n= 77) and chronic hepatitis B (CH-B) (n = 34) by
immunohistochemical staining of liver biopsy samples.
8-OHdG positive hepatocytes were significantly higher in
patients with CH-C compared to CH-B (median 55.0 vs 18.8
cells/10(5) mum(2), P < 0.0001). The number of positive
hepatocytes significantly increased with the elevation
of serum aminotransferase levels, especially in CH-C
patients (8-OHdG vs alanine aminotransferase
(ALT)/aspartate aminotrasferase (AST) were r =
0.738/0.720 in CH-C and 0.506/0.515 in CH-B). 8-OHdG
reactivity was strongly correlated with body and hepatic
iron storage markers in CH-C (vs serum ferritin, r =
0.615; vs hepatic total iron score, r = 0.520; vs
hepatic hepcidin mRNA levels, r = .571), although it was
related to serum HBV-DNA titers (r = 0.540) and age of
patients (r = -0.559) in CH-B. These results indicate
that hepatic oxidative DNA damage is common in chronic
viral hepatitis, in particular chronic HCV-infected
patients, suggesting a possible link between chronic
hepatic inflammation and hepatocarcinogenesis. The
strong positive correlation between hepatic DNA damage
and iron overload suggests that iron content is one of
the most likely mediators of hepatic oxidative stress
and iron reduction may be beneficial to reduce the
incidence of hepatic cancer in CH-C patients. Journal of
viral hepatitis [J Viral Hepat]
--------------------------------------------------------------
-------------------
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk