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Taka
Fri, Feb-01-08, 05:16
Lipids. 1997 May;32(5):535-41.

Lipid peroxidation during n-3 fatty acid and vitamin E
supplementation in humans.

Allard JP, Kurian R, Aghdassi E, Muggli R, Royall D.
Department of Medicine, University of Toronto, Ontario,
Canada.

The purpose of this study was to investigate in healthy humans
the effect of eicosapentaenoic acid (EPA) and docosahexaenoic
acid (DHA) intake, alone or in combination with
dL-alpha-tocopherol acetate (vitamin E) supplements on lipid
peroxidation. Eighty men were randomly assigned in a
double-blind fashion to take daily for 6 wk either menhaden
oil (6.26 g, n-3 fatty acids) or olive oil supplements with
either vitamin E (900 IU) or its placebo. Antioxidant
vitamins, phospholipid composition, malondialdehyde (MDA), and
lipid peroxides were measured in the plasma at baseline and
week 6. At the same time, breath alkane output was measured.
Plasma alpha-tocopherol concentration increased in those
receiving vitamin E (P < 0.0001). In those supplemented with
n-3 fatty acids, EPA and DHA increased in plasma phospholipids
(P < 0.0001) and plasma MDA and lipid peroxides increased (P <
0.001 and P < 0.05, respectively). Breath alkane output did
not change significantly and vitamin E intake did not prevent
the increase in lipid peroxidation during menhaden oil
supplementation. The results demonstrate that supplementing
the diet with n-3 fatty acids resulted in an increase in lipid
peroxidation, as measured by plasma MDA release and lipid
peroxide products, which was not suppressed by vitamin E
supplementation. PMID: 9168460

neither statins will save you - even this Pharma-sponsored
study admires that fish oil creates more peroxides:

Eur J Clin Nutr. 2003 Jun;57(6):793-800.

Increased lipid peroxidation during long-term intervention
with high doses of n-3 fatty acids (PUFAs) following an acute
myocardial infarction.

Grundt H, Nilsen DW, Mansoor MA, Nord=F8y A. Department of
Clinical Chemistry, Central Hospital in Rogaland, POB 8100,
4068 Stavanger, Norway.

OBJECTIVE: To assess the oxidative burden of a highly
concentrated compound of n-3 PUFAs as compared to corn oil by
measuring thiobarbituric acid-malondialdehyde complex
(TBA-MDA) by HPLC. We also studied the influence on TBA-MDA of
statins combined with n-3 PUFAs or corn oil. DESIGN: A
prospective, randomised, double-blind, controlled study.
SETTING: One hospital centre in Stavanger, Norway. SUBJECTS: A
total of 300 subjects with an acute myocardial infarction
(MI). INTERVENTIONS: Gelatine capsules, containing 850-882 mg
EPA and DHA as concentrated ethylesters, or 1 g of corn oil,
were ingested in a dose of two capsules twice a day for at
least 1 y. Alpha-tocopherol (4 mg) was added to all capsules
to protect the PUFAs against oxidation. RESULTS: After 1 y
TBA-MDA increased modestly in the n-3 PUFA group (n=3D125), as
compared to the corn oil group (n=3D130), P=3D0.027. Multiple
linear regression analyses of fatty acids in serum total
phospholipids (n=3D56) on TBA-MDA measured after 12 months
intervention, showed no dependency. Performing best subsets
regression, serum phospholipid concentration of arachidonic
acid (20:4 n-6 PUFA) was identified as a predictor of TBA-MDA
at 12 months follow-up, P=3D0.004.We found no impact of
statins on TBA-MDA. CONCLUSION: TBA-MDA increased modestly
after long-term intervention with n-3 PUFAs compared to corn
oil post- MI, suggesting biological changes induced by n-3
PUFAs, rather than simply reflecting their concentration
differences. The peroxidative potential of n-3 PUFAs was not
modified by statin treatment. SPONSORSHIP:: Pharmacia A/S and
Pronova A/S, Norway. PMID: 12792664

The only thing which greatly boosts the antioxidant defenses
and can protect you is CR or the low carb regime.

Taka

Mr. Natura
Thu, Feb-07-08, 16:15
Your post proves absolutely nothing.

Just thought that you might want to know.

Taka
Tue, Mar-04-08, 05:15
FASEB J. 1999 Dec;13(15):2138-42.

The effect of increased intakes of polyunsaturated fatty acids
and vitamin E on DNA damage in human lymphocytes.

Jenkinson AM, Collins AR, Duthie SJ, Wahle KW, Duthie GG.
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.

The effect of increasing dietary intakes of polyunsaturated
fatty acids (PUFAs) and vitamin E on indices of oxidative DNA
damage was investigated. Twenty-one healthy male, nonsmokers
aged 28.9 +/- 1.3 years participated in a free-living, split
plot/change over trial in which half the volunteers consumed
diets containing 5% PUFA as food energy for 4 wk and, after a
10 wk washout period, consumed a 15% PUFA diet for another 4
wk. The other volunteers followed an identical protocol,
except that they consumed the 15% PUFA diet first. The diets
were provided to volunteers either with or without an
additional 80 mg dalpha-tocopherol acetate/day; otherwise
total fat, carbohydrates, protein, and basal vitamin E
contents remained unchanged. DNA damage induced by 200 microM
H(2)O(2) in lymphocytes from volunteers as well as endogenous
DNA damage in the form of oxidized pyrimidines, measured by
alkaline single-cell gel electrophoresis (the comet assay),
significantly decreased after consumption of the 5% PUFA diet
(P<0.001 and P=0.01, respectively), but significantly
increased after consumption of the 15% PUFA diet when
alpha-tocopherol levels were in the range of 5-7 mg/day (P=0.
008 and P=0.03, respectively). These changes were abolished
by an additional 80 mg dalpha-tocopherol/day. This study
indicates that increasing dietary levels of PUFA to 15% may
adversely affect some indices of DNA stability. However,
increasing the dietary intake of vitamin E by 80 mg/day
ameliorates the damaging effects of PUFA. -Jenkinson, A.
McE., Collins, A. R., Duthie, S. J., Wahle, K. W. J., Duthie,
G. G. The effect of increased intakes of polyunsaturated
fatty acids and vitamin E on DNA damage in human lymphocytes.
PMID: 10593860

Dz
Tue, Mar-04-08, 16:16
Taka wrote: [...]

Check this out

"Coenzyme Q supplementation protects from age-related DNA
double-strand breaks and increases lifespan in rats fed on a
PUFA-rich diet"

http://hera.ugr.es/doi/15059431.pdf

Taka
Wed, Mar-05-08, 05:15
On Mar 5, 12:49 am, DZ
<24...@17712697.2376529560.29243.19108.27835> wrote:
> Taka wrote:
>
> [...]
>
> Check this out
>
> "Coenzyme Q supplementation protects from age-related DNA
> double-strand breaks and increases lifespan in rats fed on a
> PUFA-rich diet"
>
> http://hera.ugr.es/doi/15059431.pdf

That's a nice study (finally some lifelong PUFA feeding
experiment !!!) but too shortsighted. If preventing lipid
peroxidation with CoQ can increase the maximum life span by
24% imagine what a simple dietary PUFA restriction would do?
I bet you would see the same or even better effect. Too bad
they did not include the SFA control. Also what they used was
just 8% fat with 61% PUFAs. Compare it to the standard
western diet which is at least 15% fat with the same
percentage or more PUFAs!

Taka