Editor Www
Mon, Jan-28-08, 06:15
qPCR NEWS - January 2008 - Special Topic: High Resolution Melt
Dear researcher, dear Gene Quantification page reader,
Our newsletter informs about the latest news in quantitative
real-time PCR (qPCR and qRT-PCR), which are compiled and
summarised on the Gene Quantification homepage. The focus of
this newsletter issue is:
- HRM - High Resolution Melt
- updates on qPCR INFOPORTAL
- qPCR application workshops in 2008
--------------------------------------------------------------
---------------=
----
High Resolution Melting (HRM)
HRM is a novel, homogeneous, close-tube, post-PCR method,
enabling genomic researchers to analyze genetic variations
(SNPs, mutations, methylations) in PCR amplicons. It goes
beyond the power of classical melting curve analysis by
allowing to study the thermal denaturation of a
double-stranded DNA in much more detail and with much higher
information yield than ever before. HRM characterizes nucleic
acid samples based on their disassociation (melting) behavior.
Samples can be discriminated according to their sequence,
length, GC content or strand complementarity. Even single base
changes such as SNPs (single nucleotide polymorphisms) can be
readily identified. The most important High Resolution Melting
application is gene scanning - the search for the presence of
unknown variations in PCR amplicons prior to or as an
alternative to sequencing. Mutations in PCR products are
detectable by High Resolution Melting because they change the
shape of DNA melting curves. A combination of new-generation
DNA dyes, high-end instrumentation and sophisticated analysis
software allows to detect these changes and to derive
information about the underlying sequence constellation.
http://HRM.gene-quantification.info/
HRM Applications The introduction of HRM has renewed interest
in the utility of DNA melting for a wide range of uses,
including:
- Mutation discovery (gene scanning)
- Screening for loss of heterozygosity
- DNA fingerprinting
- SNP genotyping
- Characterization of haplotype blocks
- DNA methylation analysis
- DNA mapping
- Species identification
- Somatic acquired mutation ratios
- HLA compatibility typing
- Association (case/control) studies
- Allelic prevalence in a population
- Identification of candidate predisposition genes
With HRM, these and other applications are done using low-cost
generic dyes where previously custom labeled probes such as
TaqMan=AE or fluorescence resonance energy transfer (FRET)
probes were required. HRM is thus a simpler and much more
cost-effective way to characterize samples.
--------------------------------------------------------------
---------------=
----
http://HRM.gene-quantification.info/
- Introduction
- Workflow
- HRM assay design manual
- Mutation Scanning by High Resolution Melt Analysis:
Evaluation of Rotor-Gene 6000, HR-1 and 384-well
LightScanner
- High Resolution Melts - TALKs
- HRM Dyes: LC Green SYTO9 Eva Green BEBO
- Genotyping with PCR - How to choose the right approach (by
Tevik Dorak in The Scientist)
- High-Resolution Melting Curve Analysis Novel Strategies For
PCR- Based Mutation Scanning and Genotyping (by Michael
Hoffmann, Karine Raymond and Jochen Hurlebaus)
- Assay: High-Resolution Melting & Unlabeled Probes (by GEN)
- Mutation Scanning of the Cytidine Deaminase Gene by
High-Resolution Melting Curve Analysis Using the LightCycler
480 System (by Roche Applied Science in Bionity.com)
- HRM (by LTF Labortechnik)
- HRM application papers
- Further HRM publications by Roche Applied Science & Corbett
Life Science
--------------------------------------------------------------
---------------=
----
With the new qPCR INFO PORTAL and all the presented tools we
will help you with to find the right information about qPCR
and related topics in Molecular Biology in the literature and
in the World Wide Web.
=3D> Papers / Protocols / Methods / Databases / Alets / Feeds
= / Books /
Forums / E-mail / Directory
http://infoportal.gene-quantification.info/
--------------------------------------------------------------
---------------=
----
Upcoming Events World-wide academic and commercial qPCR Events
http://events.gene-quantification.info/
Symposia, Meetings, Conferences, Workshops, Seminars,
Online-Seminars, qPCR Education Program, ...etc... Please
submit your qPCR event here =3D> events@gene-
quantification.info
--------------------------------------------------------------
---------------=
----
WORKSHOP
TATAA Biocenter Germany - qPCR Application workshops
At the TATAA Biocenter Germany we offer qPCR application
workshops, the 3-day Core Module and a 2-day Biostatistics
Module. qPCR courses are held in regularly in G=F6teborg,
Sweden, in English and in Freising- Weihenstephan, Germany, in
German and English, and in Prague, Czech Republic in English
and Czech. Depending on the occasion the workshop language and
the different prices may apply. Further customized workshops
and specialized trainings will be held as well across Europe
and world-wide. TATAA Biocenter Germany courses are held in
cooperation with the Institute of Physiology, located at the
Technical University of Munich, in Freising-Weihenstephan,
near Munich, very close to the Munich Airport (MUC). For more
information and to register for the qPCR application
workshops, please see our web page:
http://tataa.gene-quantification.info/
Course Occasions 2008: 3-day qPCR Core Module (Mon. - Wed.)
and 2-day BioStatistics Module (Thu. - Fri.)
* 3rd - 7th March 2008 (in Freising, Germany, English
language)
* 5 - 9th May 2008 (in Freising, Germany, Kurs wird in
DEUTSCH gehalten, German language)
* 7 - 11th July 2008 (in Freising, Germany, English
language)
Please register here =3D>
http://www.tataa.com/Courses/Courses.html
--------------------------------------------------------------
---------------=
----
Forward Please send the qPCR NEWS to further scientists and
friends who are interested in qPCR !
Best regards,
Michael W. Pfaffl responsible Editor of the Gene
Quantification Pages http://www.gene-quantification.info
--------------------------------------------------------------
---------------=
----
If this newsletter is not displayed correctly by your email
client, please use following
http://qpcrnews.gene-quantification.info/
The qPCR NEWS and the Gene Quantification Pages are
educational sites with the only purpose of facilitating access
to qPCR related information on the internet. The qPCR NEWS and
the Gene Quantification Pages are edited by Michael W. Pfaffl
and powered by BioScience Events. Copyright =A9 2005 - 2008
All rights reserved. Any unauthorized use, reproduction, or
transfer of this message or its contents, in any medium, is
strictly prohibited. Disclaimer & Copyrights are displayed on
the homepage www.gene-quantification.com To subscribe or
change your e-mail address in qPCR NEWS, and if you would like
to receive future issues FREE of charge, please send an e-
mail with the subject SUBSCRIBE to mailto:newsletter@gene-
quantification.info?subject=3DSUBSCRIBE
Dear researcher, dear Gene Quantification page reader,
Our newsletter informs about the latest news in quantitative
real-time PCR (qPCR and qRT-PCR), which are compiled and
summarised on the Gene Quantification homepage. The focus of
this newsletter issue is:
- HRM - High Resolution Melt
- updates on qPCR INFOPORTAL
- qPCR application workshops in 2008
--------------------------------------------------------------
---------------=
----
High Resolution Melting (HRM)
HRM is a novel, homogeneous, close-tube, post-PCR method,
enabling genomic researchers to analyze genetic variations
(SNPs, mutations, methylations) in PCR amplicons. It goes
beyond the power of classical melting curve analysis by
allowing to study the thermal denaturation of a
double-stranded DNA in much more detail and with much higher
information yield than ever before. HRM characterizes nucleic
acid samples based on their disassociation (melting) behavior.
Samples can be discriminated according to their sequence,
length, GC content or strand complementarity. Even single base
changes such as SNPs (single nucleotide polymorphisms) can be
readily identified. The most important High Resolution Melting
application is gene scanning - the search for the presence of
unknown variations in PCR amplicons prior to or as an
alternative to sequencing. Mutations in PCR products are
detectable by High Resolution Melting because they change the
shape of DNA melting curves. A combination of new-generation
DNA dyes, high-end instrumentation and sophisticated analysis
software allows to detect these changes and to derive
information about the underlying sequence constellation.
http://HRM.gene-quantification.info/
HRM Applications The introduction of HRM has renewed interest
in the utility of DNA melting for a wide range of uses,
including:
- Mutation discovery (gene scanning)
- Screening for loss of heterozygosity
- DNA fingerprinting
- SNP genotyping
- Characterization of haplotype blocks
- DNA methylation analysis
- DNA mapping
- Species identification
- Somatic acquired mutation ratios
- HLA compatibility typing
- Association (case/control) studies
- Allelic prevalence in a population
- Identification of candidate predisposition genes
With HRM, these and other applications are done using low-cost
generic dyes where previously custom labeled probes such as
TaqMan=AE or fluorescence resonance energy transfer (FRET)
probes were required. HRM is thus a simpler and much more
cost-effective way to characterize samples.
--------------------------------------------------------------
---------------=
----
http://HRM.gene-quantification.info/
- Introduction
- Workflow
- HRM assay design manual
- Mutation Scanning by High Resolution Melt Analysis:
Evaluation of Rotor-Gene 6000, HR-1 and 384-well
LightScanner
- High Resolution Melts - TALKs
- HRM Dyes: LC Green SYTO9 Eva Green BEBO
- Genotyping with PCR - How to choose the right approach (by
Tevik Dorak in The Scientist)
- High-Resolution Melting Curve Analysis Novel Strategies For
PCR- Based Mutation Scanning and Genotyping (by Michael
Hoffmann, Karine Raymond and Jochen Hurlebaus)
- Assay: High-Resolution Melting & Unlabeled Probes (by GEN)
- Mutation Scanning of the Cytidine Deaminase Gene by
High-Resolution Melting Curve Analysis Using the LightCycler
480 System (by Roche Applied Science in Bionity.com)
- HRM (by LTF Labortechnik)
- HRM application papers
- Further HRM publications by Roche Applied Science & Corbett
Life Science
--------------------------------------------------------------
---------------=
----
With the new qPCR INFO PORTAL and all the presented tools we
will help you with to find the right information about qPCR
and related topics in Molecular Biology in the literature and
in the World Wide Web.
=3D> Papers / Protocols / Methods / Databases / Alets / Feeds
= / Books /
Forums / E-mail / Directory
http://infoportal.gene-quantification.info/
--------------------------------------------------------------
---------------=
----
Upcoming Events World-wide academic and commercial qPCR Events
http://events.gene-quantification.info/
Symposia, Meetings, Conferences, Workshops, Seminars,
Online-Seminars, qPCR Education Program, ...etc... Please
submit your qPCR event here =3D> events@gene-
quantification.info
--------------------------------------------------------------
---------------=
----
WORKSHOP
TATAA Biocenter Germany - qPCR Application workshops
At the TATAA Biocenter Germany we offer qPCR application
workshops, the 3-day Core Module and a 2-day Biostatistics
Module. qPCR courses are held in regularly in G=F6teborg,
Sweden, in English and in Freising- Weihenstephan, Germany, in
German and English, and in Prague, Czech Republic in English
and Czech. Depending on the occasion the workshop language and
the different prices may apply. Further customized workshops
and specialized trainings will be held as well across Europe
and world-wide. TATAA Biocenter Germany courses are held in
cooperation with the Institute of Physiology, located at the
Technical University of Munich, in Freising-Weihenstephan,
near Munich, very close to the Munich Airport (MUC). For more
information and to register for the qPCR application
workshops, please see our web page:
http://tataa.gene-quantification.info/
Course Occasions 2008: 3-day qPCR Core Module (Mon. - Wed.)
and 2-day BioStatistics Module (Thu. - Fri.)
* 3rd - 7th March 2008 (in Freising, Germany, English
language)
* 5 - 9th May 2008 (in Freising, Germany, Kurs wird in
DEUTSCH gehalten, German language)
* 7 - 11th July 2008 (in Freising, Germany, English
language)
Please register here =3D>
http://www.tataa.com/Courses/Courses.html
--------------------------------------------------------------
---------------=
----
Forward Please send the qPCR NEWS to further scientists and
friends who are interested in qPCR !
Best regards,
Michael W. Pfaffl responsible Editor of the Gene
Quantification Pages http://www.gene-quantification.info
--------------------------------------------------------------
---------------=
----
If this newsletter is not displayed correctly by your email
client, please use following
http://qpcrnews.gene-quantification.info/
The qPCR NEWS and the Gene Quantification Pages are
educational sites with the only purpose of facilitating access
to qPCR related information on the internet. The qPCR NEWS and
the Gene Quantification Pages are edited by Michael W. Pfaffl
and powered by BioScience Events. Copyright =A9 2005 - 2008
All rights reserved. Any unauthorized use, reproduction, or
transfer of this message or its contents, in any medium, is
strictly prohibited. Disclaimer & Copyrights are displayed on
the homepage www.gene-quantification.com To subscribe or
change your e-mail address in qPCR NEWS, and if you would like
to receive future issues FREE of charge, please send an e-
mail with the subject SUBSCRIBE to mailto:newsletter@gene-
quantification.info?subject=3DSUBSCRIBE