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Ironjustic
Fri, Nov-02-07, 17:16
BRAIN

Quantitative Assessment of Iron Accumulation in the Deep
Gray Matter of Multiple Sclerosis by Magnetic Field
Correlation Imaging
Y. Gea, J.H. Jensena, H. Lua, J.A. Helperna, L. Milesa,
M. Inglesea,
Y.A. Babba, J. Herbertb and R.I. Grossmana a Center for
Biomedical Imaging, Department of Radiology, New York
University Medical Center, New York, NY b Department of
Neurology, MS Care Center, Hospital for Joint Diseases,
New York University School of Medicine, New York, NY

Please address correspondence to Yulin Ge, MD, Department of
Radiology/ Center for Biomedical Imaging, New York University
School of Medicine, 650 First Ave, 6th Floor, Room 615, New
York, NY 10016; e-mail: yulin.ge@med.nyu.edu

BACKGROUND AND PURPOSE: Deposition of iron has been recognized
recently as an important factor of pathophysiologic change
including neurodegenerative processes in multiple sclerosis
(MS). We propose that there is an excess accumulation of iron
in the deep gray matter in patients with MS that can be
measured with a newly developed quantitative MR
technique-magnetic field correlation (MFC) imaging.

MATERIALS AND METHODS: With a 3T MR system, we studied 17
patients with relapsing-remitting MS and 14 age-matched
healthy control subjects. We acquired MFC imaging using an
asymmetric single-shot echo- planar imaging sequence. Regions
of interest were selected in both deep gray matter and white
matter regions, and the mean MFC values were compared between
patients and controls. We also correlated the MFC data with
lesion load and neuropsychologic tests in the patients.

RESULTS: MFC measured in the deep gray matter in patients with
MS was significantly higher than that in the healthy controls
(P .03), with an average increase of 24% in the globus
pallidus, 39.5% in the putamen, and 30.6% in the thalamus. The
increased iron deposition measured with MFC in the deep gray
matter in the patients correlated positively with the total
number of MS lesions (thalamus: r = 0.61, P
= .01; globus pallidus: r = 0.52, P = .02). A moderate but
= significant
correlation between the MFC value in the deep gray matter and
the neuropsychologic tests was also found.

CONCLUSION: Quantitative measurements of iron content with MFC
demonstrate increased accumulation of iron in the deep gray
matter in patients with MS, which may be associated with the
disrupted iron outflow pathway by lesions. Such abnormal
accumulation of iron may contribute to neuropsychologic
impairment and have implications for neurodegenerative
processes in MS.

http://www.ajnr.org/cgi/content/full/28/9/1639

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore! http://tinyurl.com/a3cc3

DEAD PEOPLE WALKING http://tinyurl.com/zk9fk

Ironjustic
Mon, Nov-05-07, 06:15
>> On Nov 2, 8:27 am, ironjustice <ironjust...@cashette.com>
>> wrote: abnormal accumulation of iron <<

<<snip>> DFO reduces disruption of the BBB <<snip>>

Ophthalmic Res. 1994;26(5):310-23. Related Articles, Links

Conjugated deferoxamine reduces blood-brain barrier disruption
in experimental optic neuritis.

Guy J, McGorray S, Qi X, Fitzsimmons J, Mancuso A, Rao N.

Department of Ophthalmology, University of Florida,
Gainesville.

The purpose of this paper was to investigate the role of
deferoxamine (DFO) scavenging of hydroxyl radical (.OH) on
disruption of the blood-brain barrier (BBB) and demyelination
in experimental optic neuritis. Eighteen strain-13 guinea pigs
were sensitized for experimental allergic encephalomyelitis.
Nine animals received 100 mg/kg of hydroxyethyl
starch-conjugated (HES) DFO by daily intraperitoneal injection
commencing the day of antigenic sensitization. Nine paired
litter mates received daily IP injections of HES. Serial fat-
suppressed magnetic resonance imaging of the optic nerves was
obtained with a T2 weighting
(T2w) to evaluate demyelination and after intravascular
administration of Gd-DTPA to evaluate BBB disruption.
The intensity of Gd-DTPA enhancement and T2w signal of
the optic nerves was quantitated 3, 7, 10 and 14 days
after antigenic sensitization. Animals were then
sacrificed and the optic nerves processed for light and
transmission electron microscopy with ultracytochemical
localization of endogenous hydrogen peroxide (H2O2) and
immunogold colocalization of extravasated serum albumin.
The area of the optic nerve head, intensity of toluidine
blue staining, and the cellular infiltrate were
digitized and quantitated. Administration of HES-DFO
significantly reduced the intensity of Gd-DTPA
enhancement in the optic nerves of HES-DFO- treated
animals compared to paired control HES animals (p =
0.0236), with the mean difference between control and
treated animals of 19.39. The difference in T2w signal
was not significant (p = 0.39), with a mean difference
between control and treated animals of -5.51. The
intensity of toluidine blue staining of optic nerve
specimens was slightly less with HES-DFO compared to
untreated animals (mean pair difference 2.48), and the
inflammatory infiltrate was reduced with HES-DFO
compared to untreated animals (mean pair difference =
61.57); these differences were not statistically
significant. In the optic nerve specimens of both groups
cerium perhydroxide-derived H2O2 reaction product was
evident in a predominantly perivascular and perineural
distribution. Immunogold- labeled serum albumin showed
extravasation at foci of perivascular inflammation in
both the presence and absence of H2O2-derived reaction
product. Conjugated DFO reduces disruption of the BBB,
as measured by Gd-DTPA enhancement, suggesting the .OH
radical generated from perivascular H2O2 may play a role
in alterations of vascular permeability in experimental
optic neuritis.

PMID: 7533278 [PubMed - indexed for MEDLINE]

--------------------------------------------------------------
-------------

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore! http://tinyurl.com/a3cc3

DEAD PEOPLE WALKING http://tinyurl.com/zk9fk

> BRAIN
>
> Quantitative Assessment of Iron Accumulation in the Deep
> Gray Matter of Multiple Sclerosis by Magnetic Field
> Correlation Imaging
> Y. Gea, J.H. Jensena, H. Lua, J.A. Helperna, L. Milesa, M.
> Inglesea,
> J.S. Babba, J. Herbertb and R.I. Grossmana a Center for
> Biomedical Imaging, Department of Radiology, New York
> University Medical Center, New York, NY b Department of
> Neurology, MS Care Center, Hospital for Joint Diseases,
> New York University School of Medicine, New York, NY
>
> Please address correspondence to Yulin Ge, MD, Department of
> Radiology/ Center for Biomedical Imaging, New York
> University School of Medicine, 650 First Ave, 6th Floor,
> Room 615, New York, NY 10016; e-mail: yulin...@med.nyu.edu
>
> BACKGROUND AND PURPOSE: Deposition of iron has been
> recognized recently as an important factor of
> pathophysiologic change including neurodegenerative
> processes in multiple sclerosis (MS). We propose that there
> is an excess accumulation of iron in the deep gray matter in
> patients with MS that can be measured with a newly developed
> quantitative MR technique-magnetic field correlation (MFC)
> imaging.
>
> MATERIALS AND METHODS: With a 3T MR system, we studied 17
> patients with relapsing-remitting MS and 14 age-matched
> healthy control subjects. We acquired MFC imaging using an
> asymmetric single-shot echo- planar imaging sequence.
> Regions of interest were selected in both deep gray matter
> and white matter regions, and the mean MFC values were
> compared between patients and controls. We also correlated
> the MFC data with lesion load and neuropsychologic tests in
> the patients.
>
> RESULTS: MFC measured in the deep gray matter in patients
> with MS was significantly higher than that in the healthy
> controls (P .03), with an average increase of 24% in the
> globus pallidus, 39.5% in the putamen, and 30.6% in the
> thalamus. The increased iron deposition measured with MFC in
> the deep gray matter in the patients correlated positively
> with the total number of MS lesions (thalamus: r = 0.61, P
> = .01; globus pallidus: r = 0.52, P = .02). A moderate but
> = significant
> correlation between the MFC value in the deep gray matter
> and the neuropsychologic tests was also found.
>
> CONCLUSION: Quantitative measurements of iron content with
> MFC demonstrate increased accumulation of iron in the deep
> gray matter in patients with MS, which may be associated
> with the disrupted iron outflow pathway by lesions. Such
> abnormal accumulation of iron may contribute to
> neuropsychologic impairment and have implications for
> neurodegenerative processes in MS.
>
> http://www.ajnr.org/cgi/content/full/28/9/1639
>
> Who loves ya. Tom
>
> Jesus Was A Vegetarian!http://jesuswasavegetarian.7h.com
>
> Man Is A Herbivore!http://tinyurl.com/a3cc3
>
> DEAD PEOPLE WALKINGhttp://tinyurl.com/zk9fk

ferrous
Mon, Nov-05-07, 17:16
"cumulation of iron <<"

Which disorder of the normal iron level control is this one?

Jesus ate a mediterranean diet.

Ironjustic
Mon, Nov-05-07, 17:16
On Nov 5, 6:42 am, ferr...@paris.com wrote: "cumulation of
iron" Which disorder of the normal iron level control is
this one? <<

You don't understand .. ?

"Iron Accumulation in the Deep Gray Matter of Multiple
Sclerosis"

You don't understand .. ?

Means you are .. stupid .. ?

I think it does ..

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore! http://tinyurl.com/a3cc3

DEAD PEOPLE WALKING http://tinyurl.com/zk9fk

ferrous
Mon, Nov-05-07, 17:16
""Iron Accumulation in the Deep Gray Matter of Multiple
Sclerosis""

Has the genetic disorder which upsets the normal iron level
control been discovered yet for this?

Jesus ate a mediterranean diet.