Ironjustic
Wed, Sep-26-07, 17:16
<<snip>> Heparin and dextran sulfate .. as compared to phytic
acid <<snip>>
J Pharm Sci. 2007 Aug 17; [Epub ahead of print] Links The
interaction of heparin/polyanions with bovine, porcine, and
human growth hormone. Joshi SB, Kamerzell TJ, McNown C,
Middaugh CR. Department of Pharmaceutical Chemistry,
University of Kansas, Lawrence, Kansas 66047.
The interaction of polyanions with proteins is of potential
pharmaceutical and cellular significance. A partial
thermodynamic description of the interaction of four
representative polyanions with human, bovine, and porcine
growth hormone is described. A heparin bead- binding assay
confirms all growth hormones bind to heparin but to varying
extents. Moderate-binding constants and high ratios of bound
protein to the more extended polyanions, heparin, and dextran
sulfate were measured by isothermal titration calorimetry and
dynamic light scattering. The binding constants and ratio of
protein bound to ligand were significantly smaller for the low
molecular weight polyanions phytic acid and sucrose
octasulfate (SOS). The effect of polyanion binding on the
bovine, porcine, and human growth hormone's (hGH) structural
and colloidal stability was also explored. Heparin and dextran
sulfate inhibit porcine somatotropin (pST) and bovine
somatotropin (bST) aggregation to the greatest extent, as
compared to phytic acid and SOS, while decreasing secondary
and tertiary structural stability as measured by the
temperature dependence of their circular dichroism and
intrinsic fluorescence. Somewhat surprisingly, the polyanions
do not appear to affect the structure or stability of hGH. The
potential biological significance of growth hormone polyanion
interactions is discussed.
(c) 2007 Wiley-Liss, Inc. and the American Pharmacists
Association J Pharm Sci.
PMID: 17705152 [PubMed - as supplied by publisher]
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Ironjustic
Wed, Sep-26-07, 17:16
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"phytic acid inhibits the binding of L- and P-selectin "
European Journal of Pharmacology Volume 520, Issues 1-3, 27
September 2005, Pages 156-163
doi:10.1016/j.ejphar.2005.07.031 Copyright =A9 2005 Elsevier
B.V. All rights reserved. Neutrophil accumulation promotes
intimal hyperplasia after photochemically induced arterial
injury in mice
Masamitsu Shimazawaa, b, , , Shinji Watanabea, Kazunao Kondoa,
Hideaki Harab, Mitsuyoshi Nakashimaa and Kazuo Umemuraa
aDepartment of Pharmacology, Hamamatsu University School of
Medicine, 3600, Handa-cho, Hamamatsu 431-3124, Japan
bDepartment of Biofunctional Molecules, Gifu Pharmaceutical
University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan
Received 24 March 2005; accepted 1 July 2005. Available online
2 September 2005.
Abstract
The role of leukocytes in the pathogenesis of coronary
arterial disease has become a focus for clinical research.
The aim of this study was to determine whether neutrophil
accumulation would participate in the development of intimal
hyperplasia after endothelial injury in mice, and whether
d-myo-inositol hexakisphosphate (phytic acid) which inhibits
the binding of L- and P- selectin to sialyl LewisX could
inhibit the development of intimal hyperplasia. Endothelial
injury was inflicted in one femoral artery via the
photochemical reaction between systemically injected rose
bengal and transillumination with green light (wavelength:
540 nm). Scanning electron microscopic observation at 3 days
after the injury showed an increase in the number of
leukocytes adhering to the injury site. Histological
observation at 21 days showed that in the neutropenia group
administered anti-neutrophil antibody and in the phytic
acid-treated group the progression of intimal hyperplasia was
significantly attenuated by comparison with the corresponding
control groups.
These results suggest that neutrophil accumulation contributes
to the initiation and/or development of intimal hyperplasia
and L- and/or P- selectin may participate in their mechanisms.
Keywords: Leukocyte; Neutrophil; Intimal hyperplasia;
Selectin; Phytic acid
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Synergistic effects of L- and P-selectin in facilitating tumor
metastasis can involve non-mucin ligands and implicate
leukocytes as enhancers of metastasis
http://www.pnas.org/cgi/content/abstract/99/4/2193
Corresponding author. Department of Biofunctional Molecules,
Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu
502-8585, Japan. Tel.: +81 58 237 3931; fax: +81 58 237 5979.
European Journal of Pharmacology Volume 520, Issues 1-3, 27
September 2005, Pages 156-163 Glycobiology Pages 707-717
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Inhibition of L- and P-selectin by a rationally synthesized
novel core 2-like branched structure containing GalNAc-Lewisx
and Neu5Ac[alpha]2-3Gal[beta]1-3GalNAc sequences
http://glycob.oxfordjournals.org/cgi/content/full/8/7/707
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Comparison of the phosphorus and mineral concentrations in
bran and abraded kernel fractions of a normal barley (Hordeum
vulgare) cultivar versus four low phytic acid isolines.
Phytic acid consists of 65-80% of the total phosphorus (P) in
cereal grains. Its salts are concentrated in the germ and
aleurone layers, which are typically removed during milling.
We hypothesize that concentrations of different types of P and
minerals in milled products will be greatly altered in low
phytic acid (lpa) barleys. Seeds of cv. Harrington (control)
and four lpa isolines-lpa1-1, lpa2-1, lpa3-1, and M955-were
abraded by a laboratory method into five surface layer and
four remaining kernel fractions. Results show that phytic acid
in the four lpa lines ranged from 75% to 5% of the control.
The decrease in phytic acid P concentration was matched almost
equally by an increase in inorganic P, so that the rest of P
(the sum of all P-containing compounds other than phytic acid
P and inorganic P) and total P levels remained relatively
unchanged among the five genotypes. These trends were also
observed for the processed fractions. The major mineral
elements in barley seeds were P, K, Mg, S, and Ca, while minor
ones were Fe, Zn, Mn, Cu, and Ba. All types of P and other
minerals measured were generally concentrated in the outer
layers of the grain. Although there were substantial
differences in mineral contents of bran fractions among
genotypes, the level of phytic acid P had little effect on
mineral contents in whole or abraded kernels. One major
exception was Fe, which had the highest level in all tissues
of M955 genotype. The above findings were all confirmed by
analyzing another set of barley samples grown in a different
environment. Thus, in general, breeding lpa barleys does not
lead to reduced mineral contents in whole grains or elevated
mineral levels in milled products.
Keywords: phytic, mineral, contents, fractions, phytic acid,
mineral contents, milled products
Authored by Liu K, Peterson KL, Raboy V. National Small Grain
and Potato Germplasm Research Facilities, Agricultural
Research Service, U=2ES. Department of Agriculture, Aberdeen,
Idaho 83210, USA. keshunL@uidaho.edu
Published in J Agric Food Chem. 2007 May 30;55(11):4453-60.
Epub 2007 May 9. The full report is available online =BB a
subscription to the periodical may be required.
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J Nutr Biochem. 2001 Oct ;12 (10):595-601 12031265 [Cited: 1]
Variation of InsP(4),InsP(5) and InsP(6) levels in tissues
and biological fluids depending on dietary phytate. [My
paper] F Grases , B Simonet , R Prieto , J March Due to the
increasing interest of InsP(6) on human health, the aim of
this paper is to compare the levels of highly phosphorilated
inositols (InsP(4), InsP(5) and InsP(6)) in organs and
biological fluids of rats and to study the influence of the
presence and absence of InsP(6) in diets. Thus, for this
purpose, the variation of InsP(4), InsP(5) and InsP(6) levels
in organs and biological fluids of rats submitted to two
different diets were studied. In the AIN-76A diet no InsP(6)
was present, yet the other was a 1% InsP(6) modified diet
(AIN-76A + 1% InsP(6)). The highest InsP(4), InsP(5) and
InsP(6) levels were found to be 10-fold superior in the brain
than those found in the kidney. When the InsP(6) was
eliminated from the diet, the InsP(6) levels decreased
dramatically (97.2% in kidney, 89.8% in brain, 100% in bone,
90.5% in plasma and 98.1% in urine), the InsP(5) levels showed
an important decrease (61.2% in kidney, 45.5% in brain,
28.1% in bone, 30% in plasma and 88.6% in urine) and the
InsP(4) levels in organs only changed slightly. From these
results, it can be deduced that the majority of InsP(6)
present in the organism is of dietary origin and its
endogenous synthesis is not important. According to the
results, it can be evidenced that the endogenous synthesis
of InsP(5) can occur, besides InsP(6) can be transformed by
enzymatic dephosphorilation in InsP(5).
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