Ironjustic
Tue, Mar-06-07, 17:17
I suppose since tetracycline has been shown to be an iron
chelator .. then this study leads one to believe **iron** to
BE .. involved ..
Br J Dermatol. 2007 Mar;156(3):548-52. Links A pilot study of
the safety and efficacy of picolinic acid gel in the treatment
of acne vulgaris.Heffernan MP, Nelson MM, Anadkat MJ. Division
of Dermatology, Washington University School of Medicine, St
Louis, MO, U.S.A.
Background Cost limitations, adverse effects or lack of
efficacy limit the use of current topical therapies in mild to
moderate acne vulgaris. Objectives To determine the safety and
efficacy of picolinic acid, a novel zinc finger therapy, in
the treatment of mild to moderate acne vulgaris. Methods
Twenty subjects with mild to moderate acne vulgaris were
treated at our centre during an open-label study with 10%
picolinic acid gel (PCL-016) twice daily to the face over 12
weeks. Results Fifteen patients completed the 12-week
open-label study. A reduction of 58.2% (P < 0.001) in mean
total lesion count,
55.5% (P < 0.001) in mean inflammatory lesion count and
59.7% (P < =2E005) in noninflammatory lesion count was
seen in this population. No serious adverse events or
clinically significant changes in laboratory values
were noted. Conclusions Results from this study suggest
that 10% picolinic acid gel applied twice daily may be
safe and effective in the treatment of mild to moderate
acne vulgaris.
PMID: 17300246 [PubMed - in process] -------------------------
---------------------------------------------------=
----------------
PMID- 7772018 OWN - NLM STAT- MEDLINE DA - 19950705 DCOM-
19950705 LR - 20041117 PUBM- Print IS - 0264-6021 VI - 308 (
Pt 2) DP - 1995 Jun 1 TI - Effect of intracellular iron
depletion by picolinic acid on expression of the lactoferrin
receptor in the human colon carcinoma cell subclone
HT29-18-C1. PG - 391-7 AB - A lactoferrin receptor has been
found on the brush-border membrane of intestinal epithelial
cells of several species, including humans. A role for this
receptor in intestinal iron absorption, which is well
regulated in response to body iron stores, has been
proposed. We have investigated the effect of intracellular
iron depletion by picolinic acid, an iron chelator, on the
cell surface binding of human lactoferrin to human
enterocytes and its intracellular uptake, using HT29-18-C1
cells, an enterocyte-like differentiable cell line. The
confluent cells exhibited
56.8 x 10(6) specific binding sites per cell for
diferric human 125I-labelled lactoferrin with
relatively low affinity (Kd 8.4 x 10(-7)
M). The addition of picolinic acid to the culture medium
resulted in a concentration- and time-dependent
increase in lactoferrin binding that was correlated
with a decrease in intracellular iron content. The
maximum effect of picolinic acid on lactoferrin
binding (approx. 2-fold increase), which appeared
between 12 and 18 h after its addition, was obtained
at a picolinic acid concentration of 2 mM. Scatchard
analysis showed that the enhanced lactoferrin binding
resulted from an increase in the number of lactoferrin
receptors rather than an alteration in the binding
affinity for lactoferrin. The time-dependent effect of
picolinic acid was completely abolished in the
presence of 1 microM anisomycin, a protein synthesis
inhibitor, indicating that ongoing protein synthesis
is involved in this effect. The enhanced lactoferrin
binding induced by picolinic acid produced an increase
of approx. 30% in the uptake of lactoferrin-bound
59Fe, indicating the existence of functional
receptors. These results suggest that biosynthesis of
lactoferrin receptors in intestinal epithelial cells
can be regulated in response to the levels of
intracellular chelatable iron, consistent with
intestinal iron absorption dependent on body iron
stores. AD - Laboratoire de Chimie Biologique,
Universite des Sciences et Technologies de Lille,
Villeneuve d'Ascq, France. FAU - Mikogami, T AU -
Mikogami T FAU - Marianne, T AU - Marianne T FAU -
Spik, G AU - Spik G LA - eng PT - Journal Article PL -
ENGLAND TA - Biochem J JID - 2984726R RN - 0
(Lactoferrin) RN - 0 (Picolinic Acids) RN - 0
(Receptors, Cell Surface) RN - 0 (lactoferrin
receptors) RN - 7439-89-6 (Iron) RN - 98-98-6
(picolinic acid) SB - IM MH - Biological
Transport/drug effects MH - Colonic
Neoplasms/metabolism MH - Humans MH - In Vitro MH -
Intestinal Absorption MH - Intestines/*metabolism MH -
Iron/*metabolism MH - Lactoferrin/metabolism MH -
Picolinic Acids/*pharmacology MH - Receptors, Cell
Surface/*metabolism MH - Research Support, Non-U.S.
Gov't MH - Tumor Cells, Cultured EDAT- 1995/06/01
MHDA- 1995/06/01 00:01
PST - ppublish SO - Biochem J 1995 Jun 1;308 ( Pt 2):391-7.
--------------------------------------------------------------
--------------= =AD----- <<snip>> Three tetracyclines
(tetracycline, doxycycline, and minocycline) were found to
possess iron-chelating activity in a colorimetric siderophore
assay. <<snip>>
Antimicrob Agents Chemother 2000 Mar;44(3):763-6
Iron-chelating activity of tetracyclines and its impact on the
susceptibility of Actinobacillus actinomycetemcomitans to
these antibiotics.
Grenier D, Huot MP, Mayrand D Groupe de Recherche en Ecologie
Buccale, Faculte de Medecine Dentaire, Quebec, Canada.
Daniel.Gren...@greb.ulaval.ca
Three tetracyclines (tetracycline, doxycycline, and
minocycline) were found to possess iron-chelating activity in
a colorimetric siderophore assay. Determination of MICs
indicated that the activity of doxycycline against the
periodontopathogen Actinobacillus actinomycetemcomitans was
only slightly influenced by the presence of an excess of iron
that likely saturates the antibiotic. On the other hand, the
MICs of doxycycline and minocycline were significantly lower
for A. actinomycetemcomitans cultivated under iron- poor
conditions than under iron-rich conditions.
PMID: 10681353, UI: 20145404
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
chelator .. then this study leads one to believe **iron** to
BE .. involved ..
Br J Dermatol. 2007 Mar;156(3):548-52. Links A pilot study of
the safety and efficacy of picolinic acid gel in the treatment
of acne vulgaris.Heffernan MP, Nelson MM, Anadkat MJ. Division
of Dermatology, Washington University School of Medicine, St
Louis, MO, U.S.A.
Background Cost limitations, adverse effects or lack of
efficacy limit the use of current topical therapies in mild to
moderate acne vulgaris. Objectives To determine the safety and
efficacy of picolinic acid, a novel zinc finger therapy, in
the treatment of mild to moderate acne vulgaris. Methods
Twenty subjects with mild to moderate acne vulgaris were
treated at our centre during an open-label study with 10%
picolinic acid gel (PCL-016) twice daily to the face over 12
weeks. Results Fifteen patients completed the 12-week
open-label study. A reduction of 58.2% (P < 0.001) in mean
total lesion count,
55.5% (P < 0.001) in mean inflammatory lesion count and
59.7% (P < =2E005) in noninflammatory lesion count was
seen in this population. No serious adverse events or
clinically significant changes in laboratory values
were noted. Conclusions Results from this study suggest
that 10% picolinic acid gel applied twice daily may be
safe and effective in the treatment of mild to moderate
acne vulgaris.
PMID: 17300246 [PubMed - in process] -------------------------
---------------------------------------------------=
----------------
PMID- 7772018 OWN - NLM STAT- MEDLINE DA - 19950705 DCOM-
19950705 LR - 20041117 PUBM- Print IS - 0264-6021 VI - 308 (
Pt 2) DP - 1995 Jun 1 TI - Effect of intracellular iron
depletion by picolinic acid on expression of the lactoferrin
receptor in the human colon carcinoma cell subclone
HT29-18-C1. PG - 391-7 AB - A lactoferrin receptor has been
found on the brush-border membrane of intestinal epithelial
cells of several species, including humans. A role for this
receptor in intestinal iron absorption, which is well
regulated in response to body iron stores, has been
proposed. We have investigated the effect of intracellular
iron depletion by picolinic acid, an iron chelator, on the
cell surface binding of human lactoferrin to human
enterocytes and its intracellular uptake, using HT29-18-C1
cells, an enterocyte-like differentiable cell line. The
confluent cells exhibited
56.8 x 10(6) specific binding sites per cell for
diferric human 125I-labelled lactoferrin with
relatively low affinity (Kd 8.4 x 10(-7)
M). The addition of picolinic acid to the culture medium
resulted in a concentration- and time-dependent
increase in lactoferrin binding that was correlated
with a decrease in intracellular iron content. The
maximum effect of picolinic acid on lactoferrin
binding (approx. 2-fold increase), which appeared
between 12 and 18 h after its addition, was obtained
at a picolinic acid concentration of 2 mM. Scatchard
analysis showed that the enhanced lactoferrin binding
resulted from an increase in the number of lactoferrin
receptors rather than an alteration in the binding
affinity for lactoferrin. The time-dependent effect of
picolinic acid was completely abolished in the
presence of 1 microM anisomycin, a protein synthesis
inhibitor, indicating that ongoing protein synthesis
is involved in this effect. The enhanced lactoferrin
binding induced by picolinic acid produced an increase
of approx. 30% in the uptake of lactoferrin-bound
59Fe, indicating the existence of functional
receptors. These results suggest that biosynthesis of
lactoferrin receptors in intestinal epithelial cells
can be regulated in response to the levels of
intracellular chelatable iron, consistent with
intestinal iron absorption dependent on body iron
stores. AD - Laboratoire de Chimie Biologique,
Universite des Sciences et Technologies de Lille,
Villeneuve d'Ascq, France. FAU - Mikogami, T AU -
Mikogami T FAU - Marianne, T AU - Marianne T FAU -
Spik, G AU - Spik G LA - eng PT - Journal Article PL -
ENGLAND TA - Biochem J JID - 2984726R RN - 0
(Lactoferrin) RN - 0 (Picolinic Acids) RN - 0
(Receptors, Cell Surface) RN - 0 (lactoferrin
receptors) RN - 7439-89-6 (Iron) RN - 98-98-6
(picolinic acid) SB - IM MH - Biological
Transport/drug effects MH - Colonic
Neoplasms/metabolism MH - Humans MH - In Vitro MH -
Intestinal Absorption MH - Intestines/*metabolism MH -
Iron/*metabolism MH - Lactoferrin/metabolism MH -
Picolinic Acids/*pharmacology MH - Receptors, Cell
Surface/*metabolism MH - Research Support, Non-U.S.
Gov't MH - Tumor Cells, Cultured EDAT- 1995/06/01
MHDA- 1995/06/01 00:01
PST - ppublish SO - Biochem J 1995 Jun 1;308 ( Pt 2):391-7.
--------------------------------------------------------------
--------------= =AD----- <<snip>> Three tetracyclines
(tetracycline, doxycycline, and minocycline) were found to
possess iron-chelating activity in a colorimetric siderophore
assay. <<snip>>
Antimicrob Agents Chemother 2000 Mar;44(3):763-6
Iron-chelating activity of tetracyclines and its impact on the
susceptibility of Actinobacillus actinomycetemcomitans to
these antibiotics.
Grenier D, Huot MP, Mayrand D Groupe de Recherche en Ecologie
Buccale, Faculte de Medecine Dentaire, Quebec, Canada.
Daniel.Gren...@greb.ulaval.ca
Three tetracyclines (tetracycline, doxycycline, and
minocycline) were found to possess iron-chelating activity in
a colorimetric siderophore assay. Determination of MICs
indicated that the activity of doxycycline against the
periodontopathogen Actinobacillus actinomycetemcomitans was
only slightly influenced by the presence of an excess of iron
that likely saturates the antibiotic. On the other hand, the
MICs of doxycycline and minocycline were significantly lower
for A. actinomycetemcomitans cultivated under iron- poor
conditions than under iron-rich conditions.
PMID: 10681353, UI: 20145404
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk