Ironjustic
Tue, Sep-26-06, 17:16
Phenolic-rich juice prevents DNA single-strand breakage and
cytotoxicity caused by tert-butylhydroperoxide in U937 cells:
the role of iron chelation. Garcia-Alonso FJ, Guidarelli A,
Periago MJ J Nutr Biochem. 2006 Sep 21;
The antioxidant potential of phenolic compounds is generally
linked to their ability to scavenge free radicals. However, in
addition to their radical-scavenging activity, phenolic
compounds can chelate metal ions, such as iron, to prevent
their participation in Fenton-type reactions, which lead to
the formation of free radicals. The aim of the present study
was to evaluate the ability of a phenolic-rich juice made from
grapes, cherries and berries to protect human myeloid leukemia
(U937) cells from oxidative stress caused by
tert-butylhydroperoxide (tB-OOH). Preincubation of cells with
extracts of the phenolic-rich juice at different
concentrations (0-200 muM ferulic acid equivalents) for 3 h
partially prevented cell death and abolished the DNA cleavage
induced by tB-OOH. Moreover, when preincubating cells with the
100-muM juice extract (the dose that diminished cell death by
around 50%), the partial prevention of tB-OOH-induced
formation of reactive oxygen species (ROS) and mitochondrial
permeability transition pore opening was observed. The radical
scavenger antioxidant N,N'-diphenyl-1,4-phenylene-diamine
(DPPD) and the intracellular iron chelator o-phenanthroline
(o-Phe) were also tested to know whether protective effects
depended on radical-scavenging or iron-chelating activities.
o-Phe prevented cell death, DNA cleavage and ROS generation,
whereas DPPD only prevented cell death, suggesting that
phenolics in the juice afforded protection against induced
oxidative stress, most probably by means of an iron-chelating
mechanism.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
cytotoxicity caused by tert-butylhydroperoxide in U937 cells:
the role of iron chelation. Garcia-Alonso FJ, Guidarelli A,
Periago MJ J Nutr Biochem. 2006 Sep 21;
The antioxidant potential of phenolic compounds is generally
linked to their ability to scavenge free radicals. However, in
addition to their radical-scavenging activity, phenolic
compounds can chelate metal ions, such as iron, to prevent
their participation in Fenton-type reactions, which lead to
the formation of free radicals. The aim of the present study
was to evaluate the ability of a phenolic-rich juice made from
grapes, cherries and berries to protect human myeloid leukemia
(U937) cells from oxidative stress caused by
tert-butylhydroperoxide (tB-OOH). Preincubation of cells with
extracts of the phenolic-rich juice at different
concentrations (0-200 muM ferulic acid equivalents) for 3 h
partially prevented cell death and abolished the DNA cleavage
induced by tB-OOH. Moreover, when preincubating cells with the
100-muM juice extract (the dose that diminished cell death by
around 50%), the partial prevention of tB-OOH-induced
formation of reactive oxygen species (ROS) and mitochondrial
permeability transition pore opening was observed. The radical
scavenger antioxidant N,N'-diphenyl-1,4-phenylene-diamine
(DPPD) and the intracellular iron chelator o-phenanthroline
(o-Phe) were also tested to know whether protective effects
depended on radical-scavenging or iron-chelating activities.
o-Phe prevented cell death, DNA cleavage and ROS generation,
whereas DPPD only prevented cell death, suggesting that
phenolics in the juice afforded protection against induced
oxidative stress, most probably by means of an iron-chelating
mechanism.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk