Ironjustic
Fri, Aug-11-06, 06:15
Effects of silymarin on the proliferation and glutathione
levels of peripheral blood mononuclear cells from
beta-thalassemia major patients. Fariba Alidoost, Marjan
Gharagozloo, Bahram Bagherpour, Abbas Jafarian, Seyed Ebrahim
Sajjadi, Hamid Hourfar, Behjat Moayedi Iron toxicity in
beta-thalassemia major is the main cause of oxidative stress
and cell mediated immune deficiencies. Despite indicative
signs of severe oxidative deficiencies associated with
beta-thalassemia major, such as decreased level of plasma
antioxidants and depletion of erythrocyte glutathione, little
is known about intracellular redox status of immune cells.
Since glutathione is a primary intracellular antioxidant and
plays an essential role in several functions in T cells, in
this study intracellular glutathione (GSH) levels as well as
proliferation of PHA-activated peripheral blood mononuclear
cells (PBMC) were investigated in 28 beta-thalassemia major
patients and 28 healthy age-matched individuals. Considering
the potential benefits of flavonoids in the therapy of
oxidative stress, the effects of silymarin on the GSH levels
and proliferation of PBMC from normal and thalassemia
individuals were further examined. Quantitative determination
of intracellular GSH and proliferative response of PBMC to PHA
were performed before and after 72 h incubation of PBMC with
various concentrations of silymarin (0, 5, 10, or 20 mug/ml).
Results demonstrated a significant reduction of GSH and
proliferation in beta-thalassemia major cells; however
treatment with silymarin led to restoration of both GSH levels
and PBMC proliferation in thalassemia patients. Considerably
low levels of GSH and depressed proliferative response of PBMC
in beta-thalassemia major may be responsible for the cell
mediated immune abnormalities in iron overload conditions.
Moreover, the GSH restoration and improvement of PBMC growth
by silymarin is a possible explanation for its recently
reported antioxidant and immunostimulatory activities. These
data suggest the benefit of using flavonoids to normalize
immune dysfunction in beta-thalassemia major. The
immunomodulatory effects of silymarin in beta-thalassemia
major are currently under further investigation in a double
blind clinical trial.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk
levels of peripheral blood mononuclear cells from
beta-thalassemia major patients. Fariba Alidoost, Marjan
Gharagozloo, Bahram Bagherpour, Abbas Jafarian, Seyed Ebrahim
Sajjadi, Hamid Hourfar, Behjat Moayedi Iron toxicity in
beta-thalassemia major is the main cause of oxidative stress
and cell mediated immune deficiencies. Despite indicative
signs of severe oxidative deficiencies associated with
beta-thalassemia major, such as decreased level of plasma
antioxidants and depletion of erythrocyte glutathione, little
is known about intracellular redox status of immune cells.
Since glutathione is a primary intracellular antioxidant and
plays an essential role in several functions in T cells, in
this study intracellular glutathione (GSH) levels as well as
proliferation of PHA-activated peripheral blood mononuclear
cells (PBMC) were investigated in 28 beta-thalassemia major
patients and 28 healthy age-matched individuals. Considering
the potential benefits of flavonoids in the therapy of
oxidative stress, the effects of silymarin on the GSH levels
and proliferation of PBMC from normal and thalassemia
individuals were further examined. Quantitative determination
of intracellular GSH and proliferative response of PBMC to PHA
were performed before and after 72 h incubation of PBMC with
various concentrations of silymarin (0, 5, 10, or 20 mug/ml).
Results demonstrated a significant reduction of GSH and
proliferation in beta-thalassemia major cells; however
treatment with silymarin led to restoration of both GSH levels
and PBMC proliferation in thalassemia patients. Considerably
low levels of GSH and depressed proliferative response of PBMC
in beta-thalassemia major may be responsible for the cell
mediated immune abnormalities in iron overload conditions.
Moreover, the GSH restoration and improvement of PBMC growth
by silymarin is a possible explanation for its recently
reported antioxidant and immunostimulatory activities. These
data suggest the benefit of using flavonoids to normalize
immune dysfunction in beta-thalassemia major. The
immunomodulatory effects of silymarin in beta-thalassemia
major are currently under further investigation in a double
blind clinical trial.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore! http://tinyurl.com/a3cc3
DEAD PEOPLE WALKING http://tinyurl.com/zk9fk