Ironjustic
Tue, Mar-07-06, 17:16
Chelator-Induced Dispersal and Killing of Pseudomonas
aeruginosa Cells in a Biofilm. Banin E, Brady KM, Greenberg EP
Appl Environ Microbiol. 2006 Mar ; 72(3): 2064-9
Biofilms consist of groups of bacteria attached to surfaces
and encased in a hydrated polymeric matrix. Bacteria in
biofilms are more resistant to the immune system and to
antibiotics than their free-living planktonic counterparts.
Thus, biofilm-related infections are persistent and often show
recurrent symptoms. The metal chelator EDTA is known to have
activity against biofilms of gram-positive bacteria such as
Staphylococcus aureus. EDTA can also kill planktonic cells of
Proteobacteria like Pseudomonas aeruginosa. In this study we
demonstrate that EDTA is a potent P. aeruginosa biofilm
disrupter. In Tris buffer, EDTA treatment of P. aeruginosa
biofilms results in 1,000-fold greater killing than treatment
with the P. aeruginosa antibiotic gentamicin. Furthermore, a
combination of EDTA and gentamicin results in complete killing
of biofilm cells. P. aeruginosa biofilms can form structured
mushroom-like entities when grown under flow on a glass
surface. Time lapse confocal scanning laser microscopy shows
that EDTA causes a dispersal of P. aeruginosa cells from
biofilms and killing of biofilm cells within the mushroom-like
structures. An examination of the influence of several
divalent cations on the antibiofilm activity of EDTA indicates
that magnesium, calcium, and iron protect P. aeruginosa
biofilms against EDTA treatment. Our results are consistent
with a mechanism whereby EDTA causes detachment and killing of
biofilm cells.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking
aeruginosa Cells in a Biofilm. Banin E, Brady KM, Greenberg EP
Appl Environ Microbiol. 2006 Mar ; 72(3): 2064-9
Biofilms consist of groups of bacteria attached to surfaces
and encased in a hydrated polymeric matrix. Bacteria in
biofilms are more resistant to the immune system and to
antibiotics than their free-living planktonic counterparts.
Thus, biofilm-related infections are persistent and often show
recurrent symptoms. The metal chelator EDTA is known to have
activity against biofilms of gram-positive bacteria such as
Staphylococcus aureus. EDTA can also kill planktonic cells of
Proteobacteria like Pseudomonas aeruginosa. In this study we
demonstrate that EDTA is a potent P. aeruginosa biofilm
disrupter. In Tris buffer, EDTA treatment of P. aeruginosa
biofilms results in 1,000-fold greater killing than treatment
with the P. aeruginosa antibiotic gentamicin. Furthermore, a
combination of EDTA and gentamicin results in complete killing
of biofilm cells. P. aeruginosa biofilms can form structured
mushroom-like entities when grown under flow on a glass
surface. Time lapse confocal scanning laser microscopy shows
that EDTA causes a dispersal of P. aeruginosa cells from
biofilms and killing of biofilm cells within the mushroom-like
structures. An examination of the influence of several
divalent cations on the antibiofilm activity of EDTA indicates
that magnesium, calcium, and iron protect P. aeruginosa
biofilms against EDTA treatment. Our results are consistent
with a mechanism whereby EDTA causes detachment and killing of
biofilm cells.
Who loves ya. Tom
Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com
Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore
DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking