Phytate (Myo-inositol hexakisphosphate) inhibits cardiovascular calcifications

[Ironjustic]

Front Biosci. 2006 Jan 1;11:136-42. Related Articles, Links

Phytate (Myo-inositol hexakisphosphate) inhibits
cardiovascular calcifications in rats.

Grases F, Sanchis P, Perello J, Isern B, Prieto RM,
Fernandez-Palomeque C, Fiol M, Bonnin O, Torres JJ.

University Institute of Health Sciences Research (IUNICS),
University of Balearic Islands.

Calcification is an undesirable disorder, which frequently
occurs in the heart vessels. In general, the formation of
calcific vascular lesions involves complex physicochemical and
molecular events. Calcification (hydroxyapatite) is initiated
by injury and is progressed by promoter factors and/or the
deficit of inhibitory signals. Myo-inositol hexakisphosphate
(phytate, InsP6) is found in organs, tissues and fluids of all
mammals and exhibits an important capacity as a
crystallization inhibitor of calcium salts in urine and soft
tissues. The levels found clearly depend on the dietary intake
but it can also be absorbed topically. In this paper, the
capacity of InsP6 as a potential inhibitor of cardiovascular
calcifications was assessed in Wistar rats. Three groups were
included, a control group, an InsP6 treated group (subjected
to calcinosis induction by Vitamin D and nicotine and treated
with standard cream with a 2% of InsP6 as potassium salt) and
an InsP6 non-treated group (only subjected to calcinosis
induction). All rats were fed AIN 76-A diet (a purified diet
in which InsP6 is undetectable). Animals were monitorized
every 12 hours. After 60 hours of calcinosis treatment, all
rats of the InsP6 non-treated group died and the rest were
sacrificed. Aortas and hearts were removed. A highly
significant increase in the calcium content of aorta and heart
tissue was observed in the InsP6 non-treated rats (21 +/- 1 mg
calcium / g dry aorta tissue, 10 +/- 1 mg calcium / g dry
heart tissue) when compared with controls (1.3 +/- 0.1 mg
calcium / g dry aorta tissue, 0.023 +/- 0.004 mg calcium / g
heart dry tissue) and InsP6 treated (0.9 +/- 0.2 mg calcium /
g dry aorta tissue, 0.30 +/- .03 mg calcium / g dry heart
tissue) animals. Only InsP6 non-treated rats displayed
important mineral deposits in aorta and heart. These findings
are consistent with the action of InsP6, as an inhibitor of
calcification of cardiovascular system.

PMID: 16146720 [PubMed - in process]

--------------------------------------------------------------
-------------------

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore

DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking

[outsor]

Tracing heme in a living cell: hemoglobin degradation and heme
traffic in digest cells of the cattle tick Boophilus
microplus. Lara FA, Lins U, Bechara GH, Oliveira PL.
Departamento de Bioquimica Medica, ICB, Universidade Federal
do Rio de Janeiro, Brazil. Heme is present in all cells,
acting as a cofactor in essential metabolic pathways such as
respiration and photosynthesis. Moreover, both heme and its
degradation products, CO, iron and biliverdin, have been
ascribed important signaling roles. However, limited knowledge
is available on the intracellular pathways involved in the
flux of heme between different cell compartments. The cattle
tick Boophilus microplus ingests 100 times its own mass in
blood. The digest cells of the midgut endocytose blood
components and huge amounts of heme are released during
hemoglobin digestion. Most of this heme is detoxified by
accumulation into a specialized organelle, the hemosome. We
followed the fate of hemoglobin and albumin in primary
cultures of digest cells by incubation with hemoglobin and
albumin labeled with rhodamine. Uptake of hemoglobin by digest
cells was inhibited by unlabeled globin, suggesting the
presence of receptor-mediated endocytosis. After endocytosis,
hemoglobin was observed inside large digestive vesicles.
Albumin was exclusively associated with a population of small
acidic vesicles, and an excess of unlabeled albumin did not
inhibit its uptake. The intracellular pathway of the heme
moiety of hemoglobin was specifically monitored using
Palladium-mesoporphyrin IX (Pd-mP) as a fluorescent heme
analog. When pulse and chase experiments were performed using
digest cells incubated with Pd-mP bound to globin
(Pd-mP-globin), strong yellow fluorescence was found in large
digestive vesicles 4 h after the pulse. By 8 h, the emission
of Pd-mP was red-shifted and more evident in the cytoplasm,
and at 12 h most of the fluorescence was concentrated inside
the hemosomes and had turned green. After 48 h, the Pd-mP
signal was exclusively found in hemosomes. In methanol, Pd-mP
showed maximal emission at 550 nm, exhibiting a red-shift to
665 nm when bound to proteins in vitro. The red emission in
the cytosol and at the boundary of hemosomes suggests the
presence of heme-binding proteins, probably involved in
transport of heme to the hemosome. The existence of an
intracellular heme shuttle from the digestive vesicle to the
hemosome acting as a detoxification mechanism should be
regarded as a major adaptation of ticks to a blood-feeding way
of life. To our knowledge, this is the first direct
observation of intracellular transport of heme in a living
eukaryotic cell. A similar approach, using Pd-mP fluorescence,
could be applied to study heme intracellular metabolism in
other cell types. PMID: 16081607 [PubMed - in process]

[Ironjustic]

Exactly .. HOW .. does this article you placed .. RELATE .. to
.. THIS .=2E thread .. ?

Does it say anything about .. IP6 .. ?

Does it say anything about .. atherosclerosis .. ?

Does .. it .. ?

Front Biosci. 2006 Jan 1;11:136-42. Related Articles, Links

Phytate (Myo-inositol hexakisphosphate) inhibits
cardiovascular calcifications in rats.

Grases F, Sanchis P, Perello J, Isern B, Prieto RM,
Fernandez-Palomeque

C, Fiol M, Bonnin O, Torres JJ.

University Institute of Health Sciences Research (IUNICS),
University of Balearic Islands.

Calcification is an undesirable disorder, which frequently
occurs in the heart vessels. In general, the formation of
calcific vascular lesions involves complex physicochemical and
molecular events. Calcification (hydroxyapatite) is initiated
by injury and is progressed

by promoter factors and/or the deficit of inhibitory signals.
Myo-inositol hexakisphosphate (phytate, InsP6) is found in
organs, tissues and fluids of all mammals and exhibits an
important capacity as

a crystallization inhibitor of calcium salts in urine and
soft tissues.

The levels found clearly depend on the dietary intake but it
can also be absorbed topically. In this paper, the capacity of
InsP6 as a potential inhibitor of cardiovascular
calcifications was assessed in Wistar rats. Three groups were
included, a control group, an InsP6 treated group (subjected
to calcinosis induction by Vitamin D and nicotine and treated
with standard cream with a 2% of InsP6 as potassium salt) and
an InsP6 non-treated group (only subjected to calcinosis
induction). All rats were fed AIN 76-A diet (a purified diet

in which InsP6 is undetectable). Animals were monitorized
every 12 hours. After 60 hours of calcinosis treatment, all
rats of the InsP6 non-treated group died and the rest were
sacrificed. Aortas and hearts were removed. A highly
significant increase in the calcium content of aorta and heart
tissue was observed in the InsP6 non-treated rats (21 +/- 1 mg
calcium / g dry aorta tissue, 10 +/- 1 mg calcium / g dry
heart tissue) when compared with controls (1.3 +/- 0.1 mg
calcium / g dry aorta tissue, 0.023 +/- 0.004 mg calcium / g
heart dry tissue) and InsP6 treated (0.9 +/- 0.2 mg calcium /
g dry aorta tissue, 0.30 +/- =2E03 mg calcium / g dry heart
tissue) animals. Only InsP6 non-treated rats displayed
important mineral deposits in aorta and heart. These findings
are consistent with the action of InsP6, as an inhibitor of
calcification of cardiovascular system.

PMID: 16146720 [PubMed - in process]

--------------------------------------------------------------
--------------= =AD-----

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore

DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking

[Ironjustic]

Exactly .. HOW .. does this article you placed .. RELATE .. to
.. THIS .=2E thread .. ?

Does it say anything about .. IP6 .. ?

Does it say anything about .. atherosclerosis .. ?

Does .. it .. ?

Front Biosci. 2006 Jan 1;11:136-42. Related Articles, Links

Phytate (Myo-inositol hexakisphosphate) inhibits
cardiovascular calcifications in rats.

Grases F, Sanchis P, Perello J, Isern B, Prieto RM,
Fernandez-Palomeque

C, Fiol M, Bonnin O, Torres JJ.

University Institute of Health Sciences Research (IUNICS),
University of Balearic Islands.

Calcification is an undesirable disorder, which frequently
occurs in the heart vessels. In general, the formation of
calcific vascular lesions involves complex physicochemical and
molecular events. Calcification (hydroxyapatite) is initiated
by injury and is progressed

by promoter factors and/or the deficit of inhibitory signals.
Myo-inositol hexakisphosphate (phytate, InsP6) is found in
organs, tissues and fluids of all mammals and exhibits an
important capacity as

a crystallization inhibitor of calcium salts in urine and
soft tissues.

The levels found clearly depend on the dietary intake but it
can also be absorbed topically. In this paper, the capacity of
InsP6 as a potential inhibitor of cardiovascular
calcifications was assessed in Wistar rats. Three groups were
included, a control group, an InsP6 treated group (subjected
to calcinosis induction by Vitamin D and nicotine and treated
with standard cream with a 2% of InsP6 as potassium salt) and
an InsP6 non-treated group (only subjected to calcinosis
induction). All rats were fed AIN 76-A diet (a purified diet

in which InsP6 is undetectable). Animals were monitorized
every 12 hours. After 60 hours of calcinosis treatment, all
rats of the InsP6 non-treated group died and the rest were
sacrificed. Aortas and hearts were removed. A highly
significant increase in the calcium content of aorta and heart
tissue was observed in the InsP6 non-treated rats (21 +/- 1 mg
calcium / g dry aorta tissue, 10 +/- 1 mg calcium / g dry
heart tissue) when compared with controls (1.3 +/- 0.1 mg
calcium / g dry aorta tissue, 0.023 +/- 0.004 mg calcium / g
heart dry tissue) and InsP6 treated (0.9 +/- 0.2 mg calcium /
g dry aorta tissue, 0.30 +/- =2E03 mg calcium / g dry heart
tissue) animals. Only InsP6 non-treated rats displayed
important mineral deposits in aorta and heart. These findings
are consistent with the action of InsP6, as an inhibitor of
calcification of cardiovascular system.

PMID: 16146720 [PubMed - in process]

--------------------------------------------------------------
--------------= =AD-----

Who loves ya. Tom

Jesus Was A Vegetarian! http://jesuswasavegetarian.7h.com

Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore

DEAD PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking