Olive oil saves cardiac cells; sat fat kills 'em

[George Che]

Have you had your oleate (olive oil) today? (Note that
l-carnitine also decreased cardiac cell apoptosis.)

George

Oleate prevents palmitate-induced cytotoxic stress in
cardiac myocytes

Abstract

The cytotoxicity of saturated fatty acids has been implicated
in the pathophysiology of cardiovascular disease, though their
effects on cardiac myocytes are incompletely understood. We
examined the effects of palmitate and the mono-unsaturated
fatty acid oleate on neonatal rat ventricular myocyte cell
biology. Palmitate (0.5 mM) increased oxidative stress, as
well as activation of the stress-associated protein kinases
(SAPK) p38, Erk1/2, and JNK, following 18 h and induced
apoptosis in 20% of cells after 24 h. Neither antioxidants nor
SAPK inhibitors prevented palmitate-induced apoptosis. Low
concentrations of oleate (0.1 mM) completely inhibited
palmitate-induced oxidative stress, SAPK activation, and
apoptosis. Increasing mitochondrial uptake of palmitate with
l-carnitine decreased apoptosis, while decreasing uptake with
the carnitine palmitoyl transferase-1 inhibitor perhexiline
nearly doubled palmitate-induced apoptosis. These results
support a model for palmitate-induced apoptosis, activation of
SAPKs, and protein oxidative stress in myocytes that involves
cytosolic accumulation of saturated fatty acids.

http://www.sciencedirect.com/science/journal/0006291X

[Mmu]

"George Cherry" <GWCherryHatesGreenEggsAndSpam@alum.mit.edu>
schrieb im Newsbeitrag
news:4PqdnQWVV_BD0ZbeRVn-vg@comcast.com...
> Have you had your oleate (olive oil) today? (Note that
> l-carnitine also decreased cardiac cell apoptosis.)
>
> George
>
> Oleate prevents palmitate-induced cytotoxic stress in
> cardiac myocytes
>
> Abstract
>
> The cytotoxicity of saturated fatty acids has been
> implicated in the pathophysiology of cardiovascular disease,
> though their effects on cardiac myocytes are incompletely
> understood. We examined the effects of palmitate and the
> mono-unsaturated fatty acid oleate on neonatal rat
> ventricular myocyte cell biology. Palmitate (0.5 mM)
> increased oxidative stress, as well as activation of the
> stress-associated protein kinases (SAPK) p38, Erk1/2, and
> JNK, following 18 h and induced apoptosis in 20% of cells
> after 24 h. Neither antioxidants nor SAPK inhibitors
> prevented palmitate-induced apoptosis. Low concentrations of
> oleate (0.1 mM) completely inhibited palmitate-induced
> oxidative stress, SAPK activation, and apoptosis. Increasing
> mitochondrial uptake of palmitate with l-carnitine decreased
> apoptosis, while decreasing uptake with the carnitine
> palmitoyl transferase-1 inhibitor perhexiline nearly doubled
> palmitate-induced apoptosis. These results support a model
> for palmitate-induced apoptosis, activation of SAPKs, and
> protein oxidative stress in myocytes that involves cytosolic
> accumulation of saturated fatty acids.
>
> http://www.sciencedirect.com/science/journal/0006291X
>

hi, unfortunately your link seems to lead to the
sciencedirect portal for that journal, not to the article
itself. could you post authors, date and/or the specific
issue/page numbers? thanks.

[Dr. Dickie]

"MMu" <brilhasti@gmx.net> wrote in message
news:430c6663$0$8024$3b214f66@usenet.univie.ac.at...
>
> "George Cherry" <GWCherryHatesGreenEggsAndSpam@alum.mit.edu>
> schrieb im Newsbeitrag
> news:4PqdnQWVV_BD0ZbeRVn-vg@comcast.com...
> > Have you had your oleate (olive oil) today? (Note that
> > l-carnitine also decreased cardiac cell apoptosis.)
> >
> > George
> >
> > Oleate prevents palmitate-induced cytotoxic stress in
> > cardiac myocytes
> >
> > Abstract
> >
> > The cytotoxicity of saturated fatty acids has been
> > implicated in the pathophysiology of cardiovascular
> > disease, though their effects on
cardiac
> > myocytes are incompletely understood. We examined the
> > effects of
palmitate
> > and the mono-unsaturated fatty acid oleate on neonatal rat
> > ventricular
myocyte
> > cell biology. Palmitate (0.5 mM) increased oxidative
> > stress, as well as activation of the stress-associated
> > protein kinases (SAPK) p38, Erk1/2, and JNK, following 18
> > h and induced apoptosis in 20% of cells after 24 h.
> > Neither antioxidants nor SAPK inhibitors prevented
> > palmitate-induced apoptosis. Low
concentrations
> > of oleate (0.1 mM) completely inhibited palmitate-induced
> > oxidative stress, SAPK activation, and apoptosis.
> > Increasing mitochondrial uptake of palmitate with
> > l-carnitine decreased apoptosis, while decreasing uptake
> > with the carnitine palmitoyl transferase-1 inhibitor
> > perhexiline nearly doubled palmitate-induced apoptosis.
> > These results support a model for palmitate-induced
> > apoptosis, activation of SAPKs, and protein oxidative
> > stress in myocytes that involves cytosolic accumulation of
> > saturated fatty acids.
> >
> > http://www.sciencedirect.com/science/journal/0006291X
> >
>
> hi, unfortunately your link seems to lead to the
> sciencedirect portal for that journal, not to the article
> itself. could you post authors, date and/or the specific
> issue/page numbers?
thanks.
>
>
>
Here it is.

Oleate prevents palmitate-induced cytotoxic stress in cardiac
myocytes . ARTICLE In Press, Uncorrected Proof, Available
online 22 August 2005 Thomas A. Miller, Nathan K. LeBrasseur,
Gregory M. Cote, Mario P. Trucillo, David R. Pimentel, Yasuo
Ido, Neil B. Ruderman and Douglas B. Sawyer