Doe
Fri, Nov-21-03, 19:12
Since iron seems to be the PROBLEM stemming from asbestos
{covered in iron which causes oxidative stress / free radicals
/ rust] .. the article below seems .. interesting ..? Iron III
doesn't induce inflammation but asbestos .. does. Is the
difference .. one is iron II and the other iron III ..?
Am J Physiol Lung Cell Mol Physiol. 2003 Nov 14 [Epub ahead
of print]. Related Articles, Links
Activation of p38 MAP kinase by asbestos in rat mesothelial
cells is mediated by oxidative stress.
Swain WA, O'Byrne KJ, Faux SP.
Department of Oncology, University of Leicester, Leicester,
Leicestershire, United Kingdom.
Asbestos fibres are bio persistent particles, which are
capable of stimulating chronic inflammatory responses in the
pleura of exposed individuals. Exposure of pleural mesothelial
cells, the progenitor cell of malignant mesothelioma
(MM), to asbestos induces an array of cellular responses. The
present studies investigated if the p38 mitogen activated
protein kinase cascade was induced under asbestos exposed
conditions. p38 plays a vital role in the response to
stressful stimuli and enables entry to an inflammatory
state characterised by cytokine production. Western blot
and in vitro kinase assays showed increases in both dual
phosphorylation and actual activity of p38 following
exposure to both fibrous and non-fibrous (milled)
crocidolite, in contrast, polystyrene beads and iron (III)
oxide had no such effects. In common with other
asbestos-induced events this was shown to be an oxidative
stress-sensitive effect as pre-incubation with either
N-acetyl-L- cysteine (NAC) or alpha-tocopherol (vitamin E)
ameliorated the effect. The present studies show that p38
activity is important for crocidolite-induced AP-1 DNA
binding as an inhibitor of p38, SB203580, reduced this
activity. Crocidolite-induced cytotoxicity was also
reduced with SB203580, indicating a role for p38 in
asbestos mediated cell death. Our studies suggest that p38
activity could be a crucial factor in the chronic immune
response elicited by asbestos and may represent a target
for future pharmacological intervention.
PMID: 14617514 [PubMed - as supplied by publisher]
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Who loves ya. Tom Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore DEAD
PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking
{covered in iron which causes oxidative stress / free radicals
/ rust] .. the article below seems .. interesting ..? Iron III
doesn't induce inflammation but asbestos .. does. Is the
difference .. one is iron II and the other iron III ..?
Am J Physiol Lung Cell Mol Physiol. 2003 Nov 14 [Epub ahead
of print]. Related Articles, Links
Activation of p38 MAP kinase by asbestos in rat mesothelial
cells is mediated by oxidative stress.
Swain WA, O'Byrne KJ, Faux SP.
Department of Oncology, University of Leicester, Leicester,
Leicestershire, United Kingdom.
Asbestos fibres are bio persistent particles, which are
capable of stimulating chronic inflammatory responses in the
pleura of exposed individuals. Exposure of pleural mesothelial
cells, the progenitor cell of malignant mesothelioma
(MM), to asbestos induces an array of cellular responses. The
present studies investigated if the p38 mitogen activated
protein kinase cascade was induced under asbestos exposed
conditions. p38 plays a vital role in the response to
stressful stimuli and enables entry to an inflammatory
state characterised by cytokine production. Western blot
and in vitro kinase assays showed increases in both dual
phosphorylation and actual activity of p38 following
exposure to both fibrous and non-fibrous (milled)
crocidolite, in contrast, polystyrene beads and iron (III)
oxide had no such effects. In common with other
asbestos-induced events this was shown to be an oxidative
stress-sensitive effect as pre-incubation with either
N-acetyl-L- cysteine (NAC) or alpha-tocopherol (vitamin E)
ameliorated the effect. The present studies show that p38
activity is important for crocidolite-induced AP-1 DNA
binding as an inhibitor of p38, SB203580, reduced this
activity. Crocidolite-induced cytotoxicity was also
reduced with SB203580, indicating a role for p38 in
asbestos mediated cell death. Our studies suggest that p38
activity could be a crucial factor in the chronic immune
response elicited by asbestos and may represent a target
for future pharmacological intervention.
PMID: 14617514 [PubMed - as supplied by publisher]
--------------------------------------------------------------
-------------
------
Who loves ya. Tom Jesus Was A Vegetarian!
http://jesuswasavegetarian.7h.com Man Is A Herbivore!
http://pages.ivillage.com/ironjustice/manisaherbivore DEAD
PEOPLE WALKING
http://pages.ivillage.com/ironjustice/deadpeoplewalking